Description
Within this work we address the relevance of integrating molecular techniques; particularly environmental DNA (eDNA) methods into biological monitoring programs. We focused on the Sebastes genus; a group of important fish in the Pacific Northwest of North America. They are particularly interesting from an evolutionary standpoint due to a high degree of interspecific sequence conservation paired with a high degree of intraspecific sequence variability. We used a novel whole mitochondrial genome approach to overcome these challenges and create successful targeted qPCR eDNA assays for species of particular importance. We also used a metabarcoding approach to compare molecular community diversity estimates with those made by conventional diving methods. We found that molecular techniques were highly sensitive and could detect more species overall. However, both molecular and conventional methods have their strengths. In terms of species and habitat protection, using multiple techniques together will be most impactful.
This data was collected in the territory of the Kitasoo/X'ais x'ais First Nation with the logistical support and permission of the Kitasoo/X'ais x'ais Stewardship Authority. If you download or use this data for any purpose please contact Christina Service the Wildlife Biologist and Science Coordinator at research@kxsa.ca.
[This dataset was processed using the GBIF eDNA converter tool.]
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 1,211 records.
1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Helbing C, Acharya-Patel N, Groenwold E, A. Lemay M, Clemente - Carvalho R, Morien E, Dudas S, Rubidge E, Lingyu Yang C, Coombe L, Warren R, Birol I, Frid A (2024). Comparison of Environmental DNA and SCUBA Diving Methods to Survey Keystone Rockfish Species on the Central Coast of British Columbia, Canada. Version 1.1. University of Victoria. Occurrence dataset. https://doi.org/10.5886/ekxjiu
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is University of Victoria. To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: d90cfe48-fe51-4206-b29d-f4c5cb67fa4a. University of Victoria publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Canada Biodiversity Information Facility.
Keywords
Occurrence
Contacts
- Metadata Provider ●
- Originator ●
- Point Of Contact
https://orcid.org/0000-0002-8861-1070
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
- Originator ●
- Point Of Contact
Geographic Coverage
British Columbia, Canada
| Bounding Coordinates | South West [52.43, -129.45], North East [52.84, -127.96] |
|---|
Taxonomic Coverage
N/A
| Kingdom | Animalia |
|---|---|
| Phylum | Chordata |
| Class | Actinopterygii |
| Order | Scorpaeniformes |
| Family | Sebastidae |
Additional Metadata
| Alternative Identifiers | 10.5886/ekxjiu |
|---|---|
| d90cfe48-fe51-4206-b29d-f4c5cb67fa4a | |
| https://data.canadensys.net/ipt/resource?r=edna-rockfish-uvic |