說明
Within this work we address the relevance of integrating molecular techniques; particularly environmental DNA (eDNA) methods into biological monitoring programs. We focused on the Sebastes genus; a group of important fish in the Pacific Northwest of North America. They are particularly interesting from an evolutionary standpoint due to a high degree of interspecific sequence conservation paired with a high degree of intraspecific sequence variability. We used a novel whole mitochondrial genome approach to overcome these challenges and create successful targeted qPCR eDNA assays for species of particular importance. We also used a metabarcoding approach to compare molecular community diversity estimates with those made by conventional diving methods. We found that molecular techniques were highly sensitive and could detect more species overall. However, both molecular and conventional methods have their strengths. In terms of species and habitat protection, using multiple techniques together will be most impactful.
This data was collected in the territory of the Kitasoo/X'ais x'ais First Nation with the logistical support and permission of the Kitasoo/X'ais x'ais Stewardship Authority. If you download or use this data for any purpose please contact Christina Service the Wildlife Biologist and Science Coordinator at research@kxsa.ca.
[This dataset was processed using the GBIF eDNA converter tool.]
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 1,211 筆紀錄。
亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Helbing C, Acharya-Patel N, Groenwold E, A. Lemay M, Clemente - Carvalho R, Morien E, Dudas S, Rubidge E, Lingyu Yang C, Coombe L, Warren R, Birol I, Frid A (2024). Comparison of Environmental DNA and SCUBA Diving Methods to Survey Keystone Rockfish Species on the Central Coast of British Columbia, Canada. Version 1.1. University of Victoria. Occurrence dataset. https://doi.org/10.5886/ekxjiu
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 University of Victoria。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: d90cfe48-fe51-4206-b29d-f4c5cb67fa4a。 University of Victoria 發佈此資源，並經由Canada Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence
聯絡資訊
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https://orcid.org/0000-0002-8861-1070
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地理涵蓋範圍
British Columbia, Canada
| 界定座標範圍 | 緯度南界 經度西界 [52.43, -129.45], 緯度北界 經度東界 [52.84, -127.96] |
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分類群涵蓋範圍
N/A
| Kingdom | Animalia |
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| Phylum | Chordata |
| Class | Actinopterygii |
| Order | Scorpaeniformes |
| Family | Sebastidae |
額外的詮釋資料
| 替代的識別碼 | 10.5886/ekxjiu |
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| d90cfe48-fe51-4206-b29d-f4c5cb67fa4a | |
| https://data.canadensys.net/ipt/resource?r=edna-rockfish-uvic |